Our roots go very deep into the life science and research industries with many of our first products being designed for PCR and Hi-Res Melting®. We continue to offer professional grade PCR master mixes along with our proprietary LCGreen® Plus melting dye.
Our LightScanner Master Mix brilliantly provides a pre-optimized solution specifically designed to deliver superior PCR performance for Hi-Res Melting and genotyping applications. Our master mix is compatible with all instructions featuring HRM capabilities. This master mix reduces time and saves money with easy-to-use premixed formulas. This secures downstream success by directly going from PCR to melting curve analysis and eliminates the risk of contamination.
This master mix incorporates a Taq polymerase, an antibody-based hot-start method, dNTPs, magnesium chloride, BioFire Defense’s proprietary LCGreen Plus dye, and is formulated at a 2.5x concentration. The antibody-mediated hot-start method means no enzyme pre-activation is required before or during thermocycling, translating into much faster PCR protocol times than other methods of hot-start protection. The master mix includes a concentration of magnesium that is optimized to work for almost all reactions. Additional magnesium chloride solution is provided for further optimization if required. The master mix is also compatible with most additives used to enhance amplification efficiency of high GC amplicons.
LCGreen Plus dye is specifically designed for Hi-Res Melting curve analysis to detect DNA sequence variants. The addition of LCGreen Plus dye increases the melting temperature of DNA by 1-3 °C and may require adjustment of cycling parameters. This dye is manufactured exclusively by BioFire Defense and its chemical structures are unique among the scientific and patent literature. LCGreen Plus dye is tailored specifically for Hi-Res Melting and has the unique ability to detect heteroduplexes during melting analysis after PCR. Just add the dye to your sample before PCR. LCGreen Plus dye is extremely stable, does not inhibit PCR, and is a “saturation” dye which makes it possible to detect multiple PCR products in a mixture during melting analysis.
LCGreen Plus is tailored for use in melting instruments with 96- or 384-well microtiter plates. It has superb fluorescence intensity and can be used with most fluorescence based PCR detection systems such as the Roche LightCycler instrument. For optimal performance, the use of a Hi-Res Melting instrument is suggested.